Antioxidant activity test of propolis trigona sp extract from Cibubur using the dpph (1,1-diphenyl-2 picrylhydrazil) method
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Nov 30, 2022
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Antioxidants, DPPH, Extracts of propolis Tigona Sp
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Abstract
Propolis is a resinous substance that comes from the wood and top of the plants gathered by bees, then mixed with wax and bee saliva. Propolis contains several active compounds such as flavonoids that could act as an antioxidant. The content of the active compound in propolis depends on the geographic factor, the bee species, and how to make the extract. The purpose of this research was to determine the antioxidant activity of propolis extracts Trigona sp from Cibubur with 96% ethanol using the DPPH (1,1-Diphenyl-2-Picrylhidrazyl) method. In this study the vitamin C was a positive control. The measurement of antioxidant activity used UV-Vis spectrophotometer with it's wavelength 517 nm. The results showed that the IC50 of propolis extract was 416.486 ppm while vitamin C was 5.96 ppm.
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How to Cite
Sadiqy, M. C. A. (2022). Antioxidant activity test of propolis trigona sp extract from Cibubur using the dpph (1,1-diphenyl-2 picrylhydrazil) method. International Journal on ObGyn and Health Sciences, 1(1), 10–16. Retrieved from https://trigin.pelnus.ac.id/index.php/ObGyn/article/view/56
References
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Radiati, Lilik Eka, et al. Study of Propolis, Pollen and Royal Jelly in Honey Products as Natural Antioxidants. Journal of Animal Products Science and Technology, University of Brawijaya, Pages 35 – 39, February 2007.
Cook NC, Samman S., Nutritional Biochemistry, 7, 66—76 (1996).
Ilham Kuncahyo, Sunardi. Antioxidant Activity Test of Carambola Fruit Extract (Averrhoa Bilimbi, L.) Against 1,1-Diphenyl-2-Picrylhidrazyl (Dpph). Yogyakarta: Pharmaceutical Technology, Faculty of Engineering, Setia Budi University. 2007.
Suranto, adji. The power of propolis to combat disease. Jakarta : AgroMedia Pustaka. 2010
Hegazi, AG, 1997. Propolis an overvie. International Symposium of Aphiterapy 8-9th. Cairo
The Qur'an and its translation. 2005. AL-JUMUNATUL ALI, Ministry of Religion of the Republic of Indonesia. J-ART Publisher CV. pg 275
Bankova V and Popova M. 2007. Propolis of stingless bee : a promising source of biologically active compounds. Phcog Rev 1 (1) : 82-92
Hardianty, Desi. 2011. Administration of Oral Propolis Extract Reduces F2-Isoprostane Levels in the Urine of Male White Rats (Rattus Novergicus) Experiencing Maximum Physical Activity. Postgraduate Program, Udayana University, Denpasar, Bali. Pages: 1-70.
Greenaway W, Scaysbrook T, Whatley FR. 1990. The composition and plant origins of propolis: A report of work at Oxford, Bee World. 71: 107–18.
Bankova VS. Castro SL and Marcucci MC. 2000. Propolis: recent advances in chemistry and plant origin. Apidologie 31: 3-15
Bankova VS, Milena P, Stefan B, and Anna GS.2002. Chemical composition of European propolis. Expected and Unexpected Result. Z Naturforsch 57c : 530-533
Radiati, Eka Lilik, Umi Kalsum, et al. 2008. The Effect of Propolis Extract on the Cellular Immune System in Wistar Strain White Rats (Rattus norvegicus). Faculty of animal husbandry, Brawijaya University. Vol 9. No. 1 : 1-9
Kumuzawa S, Hitomi G, Tomoko H, Syuichi F, Takunori F, and Tsutomu N. 2006. A New prenylated flavonoid from propolis collected in Okinawa, Japan. Biosci biotechnol Biochem 68 (1) : 260-262.
Marcucci MC. 1995. Propolis: Chemical composition, biological properties and therapeutic activity. Apidologie 26 : 83-99
M. Ranjith Kumar, V. Subash Chandra Bose, S. Sathyabama and V. Brindha Priyadarisini. 2011. Antimicrobial and DPPH Free Radical-Scavenging Activities of the Ethanol Extract of Propolis Collected from India. Journal of Ecobiotechnology; Vol.3, No.1.
Fatoni, Amen. 2008. Effect of Propolis Trigona Spp. The Origin of Bukit Tinggi Against Several Bacteria in the Cow's Small Intestine and Tracing of Their Active Components. Thesis, Graduate School, Bogor Agricultural Institute. Pages: 17-40.
Blouis MS. Antioxidant Determinations by The Use of a Stable Free Radical. Nature : 1958. 1199-1200.
Salatino, A., Teixera, EW, Negri, G., Dejair. 2005. Origin and Chemical Variation of Brazilian Propolis. Department of Botany. Brazil: Institute of Biosciences University of São Paulo Brazil. Published by Oxford University Press
Burdock GA 1998. Review of the biological properties and toxicity of bee Propolis. Food Chem Toxicol. 36:347–63.
Krell, R. 1996. Value-added Products from beekeeping : FAO Agricultural services Bulltein No. 124. Food and Agriculture Organization of the United Nations Rome 1996.
Hairrudin, Dina Helianti. The Protective Effect of Propolis in Preventing Oxidative Stress Due to Heavy Physical Activity (Swimming Stress). Journal of BASIC SCIENCES, Faculty of Medicine, University of Jember, Vol. 10 No. 2, Pg 207-211 July 2009.
Suhartono, E., Ujiati, Aflanie, I. Oxygen Toxicity by Radiation and Effect of Glutamic pyruvate transamine (GPT) Activity Rat plasma after vitamin C treatment. Yogyakarta: Proposed at the international seminar on Environmental Chemistry and Toxicology. 2002
Halliwell, B and Gutteridge, JMC Free radicals in biology and medicine. New York: oxford university press. 2000.
Wong D. Mechanism and theory in food chemistry. New York: Van Nostrad Reinhold. 1989.
Hernani, Rahardjo, Mono. Antioxidant Efficacious Plants. Jakarta: Independent Spreader. 2006.
Ardiansyah. Antioxidants and Their Role in Health. science and technology articles. 2007. Access 28 March 2010.
Vimala, S., Adenan, MI, AR and Shahdan Rohana. 2003. Nature's Choice to Wellness
: Antioxidant vegetables/Ulam. Malaysia, Kuala Lumpur: Forest Research Institute.
Mun'im, A, Negishi, O and Ozawa, T. 2003. Antioxidative compounds from Crotalaria sessiliflora, Biosci. Biotechnol. Biochem.. Hal 410-414.
Harborne, J.B. Phytochemical Methods, A Guide to Modern Methods of Analyzing Plants.
Translation of K. Padmawinata Edition II. Bandung: ITB Press. 2006
Gurav, SN Deshkar, V. Gulkari, N. Duragkar, A. Patil. 2007. Free radical scavenging activity of polygala chinensis linn. Pharmacologyonline, 2: 245-253.
Molyneux P. The Use of The Stable Free Radical Dipenylpicrylhydrazyl (DPPH) for Estimating antioxidant activity. Songklanakarin: Science Technology. 2004. 26 (2): 211-219.
Sroka, Zbigniew. 2006. The screening analysis of antiradical activity of some
plant extracts. Department of Pharmacognosy, Wrocław Medical University, Wrocław,
Poland. Postepy High Med Dosw. (online), 60: 563-570.
Gandjar IG, Rohman A. Analytical pharmaceutical chemistry. Yogyakarta: Student Library. 2007
Rouessac F, Rouessac A. Chemical analysis of modern instrumentation, methods and techniques. England: Willey. 2004
Lachumy SJT, Sasidharan S, Sumathy V, Zakarin Z. Pharmacological Activity, Phytochemical analysis and Toxicity of methanol extract of Etlingera elatior (Torch Ginger) Flowers. Malaysia : Asian Pacific Journal of Tropical Medicine. 2010. 769-774.
Green RJ. Antioxidant Activity of peanut plant tissues. thesis. North Caroline State University, Raleihgh : Department of food science.2004
Saputri FC, Jantan I. Effect of selected medicinal plants on human low-density lipoprotein oxidation, 2,2-diphenyl-1-picryhydrazyl (DPPH) radicals and human aggregation. Journal of Medicinal Plants Research 2011 Nov ; 5 (26) : 6182-6191
Nabavi SF, Nabavi SN, Ebrahimzadeh MA, Asgarirad H. The Antioxidant Activity of Wild Medlar (Mespilus germanical) Fruits, Stem Bark and Leaf. African Journal of Biotechnology 10 January 2011; Vol.10(2): pp. 283-289. Available from:URL: http://www.academicjournals.org/AJB
Radiati, Lilik Eka, et al. Study of Propolis, Pollen and Royal Jelly in Honey Products as Natural Antioxidants. Journal of Animal Products Science and Technology, University of Brawijaya, Pages 35 – 39, February 2007.
Cook NC, Samman S., Nutritional Biochemistry, 7, 66—76 (1996).
Ilham Kuncahyo, Sunardi. Antioxidant Activity Test of Carambola Fruit Extract (Averrhoa Bilimbi, L.) Against 1,1-Diphenyl-2-Picrylhidrazyl (Dpph). Yogyakarta: Pharmaceutical Technology, Faculty of Engineering, Setia Budi University. 2007.
Suranto, adji. The power of propolis to combat disease. Jakarta : AgroMedia Pustaka. 2010
Hegazi, AG, 1997. Propolis an overvie. International Symposium of Aphiterapy 8-9th. Cairo
The Qur'an and its translation. 2005. AL-JUMUNATUL ALI, Ministry of Religion of the Republic of Indonesia. J-ART Publisher CV. pg 275
Bankova V and Popova M. 2007. Propolis of stingless bee : a promising source of biologically active compounds. Phcog Rev 1 (1) : 82-92
Hardianty, Desi. 2011. Administration of Oral Propolis Extract Reduces F2-Isoprostane Levels in the Urine of Male White Rats (Rattus Novergicus) Experiencing Maximum Physical Activity. Postgraduate Program, Udayana University, Denpasar, Bali. Pages: 1-70.
Greenaway W, Scaysbrook T, Whatley FR. 1990. The composition and plant origins of propolis: A report of work at Oxford, Bee World. 71: 107–18.
Bankova VS. Castro SL and Marcucci MC. 2000. Propolis: recent advances in chemistry and plant origin. Apidologie 31: 3-15
Bankova VS, Milena P, Stefan B, and Anna GS.2002. Chemical composition of European propolis. Expected and Unexpected Result. Z Naturforsch 57c : 530-533
Radiati, Eka Lilik, Umi Kalsum, et al. 2008. The Effect of Propolis Extract on the Cellular Immune System in Wistar Strain White Rats (Rattus norvegicus). Faculty of animal husbandry, Brawijaya University. Vol 9. No. 1 : 1-9
Kumuzawa S, Hitomi G, Tomoko H, Syuichi F, Takunori F, and Tsutomu N. 2006. A New prenylated flavonoid from propolis collected in Okinawa, Japan. Biosci biotechnol Biochem 68 (1) : 260-262.
Marcucci MC. 1995. Propolis: Chemical composition, biological properties and therapeutic activity. Apidologie 26 : 83-99
M. Ranjith Kumar, V. Subash Chandra Bose, S. Sathyabama and V. Brindha Priyadarisini. 2011. Antimicrobial and DPPH Free Radical-Scavenging Activities of the Ethanol Extract of Propolis Collected from India. Journal of Ecobiotechnology; Vol.3, No.1.
Fatoni, Amen. 2008. Effect of Propolis Trigona Spp. The Origin of Bukit Tinggi Against Several Bacteria in the Cow's Small Intestine and Tracing of Their Active Components. Thesis, Graduate School, Bogor Agricultural Institute. Pages: 17-40.
Blouis MS. Antioxidant Determinations by The Use of a Stable Free Radical. Nature : 1958. 1199-1200.
Salatino, A., Teixera, EW, Negri, G., Dejair. 2005. Origin and Chemical Variation of Brazilian Propolis. Department of Botany. Brazil: Institute of Biosciences University of São Paulo Brazil. Published by Oxford University Press
Burdock GA 1998. Review of the biological properties and toxicity of bee Propolis. Food Chem Toxicol. 36:347–63.
Krell, R. 1996. Value-added Products from beekeeping : FAO Agricultural services Bulltein No. 124. Food and Agriculture Organization of the United Nations Rome 1996.
Hairrudin, Dina Helianti. The Protective Effect of Propolis in Preventing Oxidative Stress Due to Heavy Physical Activity (Swimming Stress). Journal of BASIC SCIENCES, Faculty of Medicine, University of Jember, Vol. 10 No. 2, Pg 207-211 July 2009.
Suhartono, E., Ujiati, Aflanie, I. Oxygen Toxicity by Radiation and Effect of Glutamic pyruvate transamine (GPT) Activity Rat plasma after vitamin C treatment. Yogyakarta: Proposed at the international seminar on Environmental Chemistry and Toxicology. 2002
Halliwell, B and Gutteridge, JMC Free radicals in biology and medicine. New York: oxford university press. 2000.
Wong D. Mechanism and theory in food chemistry. New York: Van Nostrad Reinhold. 1989.
Hernani, Rahardjo, Mono. Antioxidant Efficacious Plants. Jakarta: Independent Spreader. 2006.
Ardiansyah. Antioxidants and Their Role in Health. science and technology articles. 2007. Access 28 March 2010.
Vimala, S., Adenan, MI, AR and Shahdan Rohana. 2003. Nature's Choice to Wellness
: Antioxidant vegetables/Ulam. Malaysia, Kuala Lumpur: Forest Research Institute.
Mun'im, A, Negishi, O and Ozawa, T. 2003. Antioxidative compounds from Crotalaria sessiliflora, Biosci. Biotechnol. Biochem.. Hal 410-414.
Harborne, J.B. Phytochemical Methods, A Guide to Modern Methods of Analyzing Plants.
Translation of K. Padmawinata Edition II. Bandung: ITB Press. 2006
Gurav, SN Deshkar, V. Gulkari, N. Duragkar, A. Patil. 2007. Free radical scavenging activity of polygala chinensis linn. Pharmacologyonline, 2: 245-253.
Molyneux P. The Use of The Stable Free Radical Dipenylpicrylhydrazyl (DPPH) for Estimating antioxidant activity. Songklanakarin: Science Technology. 2004. 26 (2): 211-219.
Sroka, Zbigniew. 2006. The screening analysis of antiradical activity of some
plant extracts. Department of Pharmacognosy, Wrocław Medical University, Wrocław,
Poland. Postepy High Med Dosw. (online), 60: 563-570.
Gandjar IG, Rohman A. Analytical pharmaceutical chemistry. Yogyakarta: Student Library. 2007
Rouessac F, Rouessac A. Chemical analysis of modern instrumentation, methods and techniques. England: Willey. 2004
Lachumy SJT, Sasidharan S, Sumathy V, Zakarin Z. Pharmacological Activity, Phytochemical analysis and Toxicity of methanol extract of Etlingera elatior (Torch Ginger) Flowers. Malaysia : Asian Pacific Journal of Tropical Medicine. 2010. 769-774.
Green RJ. Antioxidant Activity of peanut plant tissues. thesis. North Caroline State University, Raleihgh : Department of food science.2004
Saputri FC, Jantan I. Effect of selected medicinal plants on human low-density lipoprotein oxidation, 2,2-diphenyl-1-picryhydrazyl (DPPH) radicals and human aggregation. Journal of Medicinal Plants Research 2011 Nov ; 5 (26) : 6182-6191
Nabavi SF, Nabavi SN, Ebrahimzadeh MA, Asgarirad H. The Antioxidant Activity of Wild Medlar (Mespilus germanical) Fruits, Stem Bark and Leaf. African Journal of Biotechnology 10 January 2011; Vol.10(2): pp. 283-289. Available from:URL: http://www.academicjournals.org/AJB
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